ApeKI

• Description:

  ApeKI is a Type IIP restriction enzyme derived from the Aeropyrum pernix K1. It recognizes and cleaves the sequence G/CWGC, generating sticky-end with a 3-base overhang. ApeKI is one of the preferred enzymes for Genotyping-by-Sequencing (GBS) and is widely used in plant and animal genetics, population evolution studies, and functional gene mapping.

• Assay Protocol:

  http://en.best-enzymes.com/storage/upload/files/directions/04xianzhimei/EG25519.pdf

• Components:

  • ApeKI (10 U/μl) : 50 μl • 10x Cut Buffer C: 1 ml

• Features & Benefits:

  Recommended Reaction Conditions 1x Cut Buffer C; Incubate at 75°C; Refer to "Protocol for DNA Digestion" for reaction setup. Heat Inactivation This enzyme can not be heat inactivated. Please purify the digested product by phenol/chloroform treatment or column-based purification kit. Methylation sensitivity Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.

• Storage Conditions:

  Store at -20°C for 2 years.

• Isoschizomers:

  TseI

• Cut Site:

  GCWGC 5'...G↓C W G C...3' 3'...C G W C↑G ...5'