Exonuclease III

• Description:

  Exonuclease III is a nuclease that stepwise removes mononucleotides from the 3'-OH termini of double-stranded DNA (dsDNA), and inactive on single-stranded DNA (ssDNA) . The preferred substrates of Exonuclease III are blunt or recessed 3'-termini, although the enzyme also acts at nick sites in double-stranded DNA to generate single-stranded gaps. 3'-overhanging termini are resistant to cleavage; the degree of resistance depends on the length of the extension, with extensions 4 bases or longer being essentially resistant to cleavage. This characteristic can be used to produce strand-specific ssDNA with designed linearized dsDNA having one non-cleavage end (3'-overhang) and the other end designed as a cleavable end (blunt or 5'-overhang), allowing Exonuclease III to digest only one strand. Exonuclease III also exhibits RNase H, 3'-phosphatase, and apurinic/apyrimidinic-endonuclease activities.

• Assay Protocol:

  http://en.best-enzymes.com/storage/upload/files/directions/05xiushimei/EG20207.pdf

• Concentration:

  100 U/μL

• Components:

  • Exonuclease III (100 U/μl) : 50 μl • 10xExo III Buffer: 500 μl

• Shipping Conditions:

  2~8°C

• Storage Conditions:

  -20°C

• Shelf Life:

  2 Years