One Step RT-qPCR Probe Kit v2

CAT:
1465-EG22109S
Size:
250 Reactions
  • Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
  • Dry Ice Shipment: No

One Step RT-qPCR Probe Kit v2

  • Description:

    One Step RT-qPCR Probe Kit v2 is a kit for multiple reverse transcription - fluorescent quantitative PCR reactions using RNA as a template. During the experiment, reverse transcription and quantitative PCR are performed in the same reaction tube, which simplifies the experimental operation and reduces the risk of contamination. The kit contains thermo-stable Reverse Transcriptase to efficiently synthesize the first-strand cDNA, and contain hot-start Ab-Taq DNA Polymerase to quantitative amplification, making it suitable for probe based qPCR. The product already includes all components for reverse transcription - fluorescent quantitative PCR, excluding primers and sample RNA, including RNase inhibitors, RTase, and Ab-Taq. It can reduce operation steps, shorten sample addition time, and lower the risk of contamination.

  • Assay Protocol:

    http://en.best-enzymes.com/storage/upload/files/directions/03yingguangdingliang/EG22109.pdf

  • Components:

    • RTQ Enzyme Mix: 250 μl • 2xRTQ Reaction Buffer: 3x1 ml

  • Shipping Conditions:

    2~8°C

  • Storage Conditions:

    -20°C

  • Shelf Life:

    1 Year