SpCas9-NLS

• Description:

  SpCas9-NLS is an RNA-guided endonuclease from Streptococcus pyogenes. Guided by crRNA and tracrRNA (or a sgRNA formed by their fusion), SpCas9-NLS recognizes the region immediately upstream of the PAM sequence (5'-NGG) in double-stranded DNA targets and produces a double-stranded break precisely three nucleotides upstream of the PAM. SpCas9-NLS is commonly employed for gene editing. To enhance cellular editing efficiency, SpCas9-NLS contains a nuclear localization sequence (NLS) derived from Simian virus 40 (SV40) T antigen on N-termini. Additionally, this product can be utilized for the cleavage of target DNA in vitro and gene cloning, etc.

• Assay Protocol:

  http://en.best-enzymes.com/storage/upload/files/directions/05xiushimei/EG24205.pdf

• Concentration:

  10 μM

• Components:

  • SpCas9-NLS (10 μM) : 100 μl • 10x Cut Buffer C: 1 ml

• Shipping Conditions:

  2~8°C

• Storage Conditions:

  Store at -20°C for 2 years.

• Shelf Life:

  2 Years