T7 High Yield RNA Synthesis Kit

• Description:

  The T7 High Yield RNA Synthesis Kit is designed for the large-scale synthesis of RNA through in vitro transcription using T7 RNA polymerase and DNA templates containing a T7 promoter. It is suitable for both long and short RNA transcripts. The T7 Enzyme Mix included RNase inhibitor and inorganic pyrophosphatase. Additionally, DNase I, RNase-free, is provided to eliminate template DNA after the transcription reaction. Using this kit, 1 μg of linearized double-stranded DNA template can yield at least 150 μg of RNA. The synthesized RNA is applicable for a wide range of downstream applications, including in vitro translation, RNA structure and function studies, RNase protection assays, probe hybridization, RNA interference, and more.

• Assay Protocol:

  http://en.best-enzymes.com/storage/upload/files/directions/16tiwaizhuanlu/EG24104.pdf

• Components:

  • T7 Enzyme Mix: 100 μl • 10x T7 RNA Pol Buffer: 100 μl • ATP (100mM) : 100 μl • UTP (100mM) : 100 μl • GTP (100mM) : 100 μl • CTP (100mM) : 100 μl • Control Template (0.5 μg/μl) : 10 μl • DNase I, RNase Free (1 U/μl) : 100 μl • Nuclease-Free Water: 1 ml

• Shipping Conditions:

  2~8°C

• Storage Conditions:

  -20°C

• Shelf Life:

  2 Years