RNase T1

• Description:

  RNase T1 is an endoribonuclease obtained from Aspergillus oryzae, which exhibits specificity for cleaving single-stranded RNA following guanosine ribonucleotides (G), resulting in the formation of 3'-phosphate termini. RNase T1 is capable of forming a nucleoside 2', 3'-cyclic phosphate intermediate to cleave the phosphodiester bond between the 3'-guanosine residue and the 5'-OH group of the adjacent nucleoside, producing oligonucleotides with terminal 3'-GMP and 3'-GMP. The activity of RNase T1 does not depend on metal ions.

• Assay Protocol:

  http://en.best-enzymes.com/storage/upload/files/directions/05xiushimei/EG24210.pdf

• Concentration:

  500 U/μL

• Components:

  • RNase T1 (500 U/μl) : 200 μl • 10x RNase T1 Buffer: 1 ml

• Shipping Conditions:

  2~8°C

• Storage Conditions:

  -20°C

• Shelf Life:

  2 Years