Thermostable RNase HII

• Description:

  This product is derived from the extremely thermophilic bacterium Pyrococcus abyssi (P.abyssi) and obtained through recombinant expression and purification. Thermostable RNase H is an endonuclease that specifically recognizes and cleaves the RNA strand in RNA/DNA heteroduplexes, without cleaving sSDNA, dsDNA, and exhibits minimal cleavage activity towards single-stranded RNA. Thermostable RNase Hll cleaves at the 5' end of ribonucleotide residues, generating a 5'-phosphate group and a 3'-hydroxyl group after cleavage. This Thermostable RNase Hll exhibits optimal activity within the temperature ranging from 60 to 80°C, remains active between 37°C and 95°C, and exhibits low activity at room temperature. The product is extremely thermostable, with almost no activity loss following incubation at 95°C for 15 minutes, making it compatible with various PCR reaction systems.

• Assay Protocol:

  http://en.best-enzymes.com/storage/upload/files/directions/05xiushimei/EG24212.pdf

• Concentration:

  2 U/μL

• Components:

  • Thermostable RNase HII (2 U/μl) : 200 μl • HII Dilution Buffer: 2x1 ml • 10x RHII Buffer: 1 ml

• Shipping Conditions:

  2~8°C

• Storage Conditions:

  -20°C

• Shelf Life:

  2 Years