T4 DNA Ligase (Fast)

• Description:

  T4 DNA Ligase (Fast) is produced by E.coli carrying T4 bacteriophage gene 30. The enzyme catalyzes the formation of phosphodiester bonds between 5'-phosphate groups and 3'-hydroxyl groups of double-stranded DNA or RNA. It can repair single-strand nicks in double-stranded DNA, RNA, or DNA/RNA hybrids and can ligate DNA fragments with sticky or blunt ends. It is not active on single-stranded nucleic acids. The enzyme is primarily used for cloning of restriction endonuclease-digested DNA fragments, site-directed mutagenesis, cloning of PCR products, linear DNA circularization, and repair of double-stranded DNA nicks. T4 DNA Ligase (Fast) requires ATP as a cofactor and completes sticky end ligation reactions at room temperature in just 10 minutes.

• Assay Protocol:

  http://en.best-enzymes.com/storage/upload/files/directions/05xiushimei/EG15205-1.pdf

• Concentration:

  5 U/μL

• Components:

  • T4 DNA Ligase (Fast) (5 U/μl) : 200 μl • 10xT4 DNA Ligase Buffer: 2x1 ml • 50% PEG: 1 ml

• Shipping Conditions:

  2~8°C

• Storage Conditions:

  Store at -20°C for 2 years.

• Shelf Life:

  2 Years