T4 RNA Ligase 1

• Description:

  T4 RNA Ligase 1 is an ATP-dependent ligase derived from bacteriophage T4. It catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3 hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond with hydrolysis of ATP to AMP and PPi. The ligation efficiency of T4 RNA Ligase 1 varies significantly for different substrates, following the order: ssRNA-ssRNA>ssRNA-ssDNA>ssDNA-ssDNA. Additionally, T4 RNA Ligase 1 can also be used for ligation between RNA and mononucleotides, provided that the mononucleotides are phosphorylated at both the 5' and 3' ends. This application is commonly used for 3'-end labeling of RNA.

• Assay Protocol:

  http://en.best-enzymes.com/storage/upload/files/directions/05xiushimei/EG25204.pdf

• Concentration:

  10 U/μL

• Components:

  • T4 RNA Ligase 1 (10 U/μl) : 500 μl • 10x T4 Rnl1 Buffer: 1.5 ml • 50% PEG 8000 (RNase-free) : 2x1 ml • ATP (10 mM) : 1 ml

• Shipping Conditions:

  2~8°C

• Storage Conditions:

  Store at -20°C for 2 years.

• Shelf Life:

  2 Years