M-MLV GIII Reverse Transcriptase

• Description:

  M-MLV GIII Reverse Transcriptase is third-generation reverse transcriptase derived through genetic engineering and recombinant technology from the Moloney Murine Leukemia Virus (M-MLV) . This enzyme has been modified to eliminate the RNase H activity. Additionally, it exhibits significantly improved thermal stability (optimal reaction temperature:50 °C) . These greatly improve the specificity and length of the synthesized first-strand cDNA, allowing for the synthesis of cDNA fragments up to 12 kb. Moreover, M-MLV GIII Reverse Transcriptase shows enhanced tolerance to RNA secondary structures.

• Assay Protocol:

  http://en.best-enzymes.com/storage/upload/files/directions/02nizhuanlu/EG15124.pdf

• Concentration:

  200 U/μL

• Components:

  • M-MLV GIII Reverse Transcriptase (200 U/μl) : 50 μl • 5x M-MLV First Strand Buffer: 500 μl • 0.1 M DTT: 100 μl

• Shipping Conditions:

  2~8°C

• Storage Conditions:

  Store at -20°C for 2 years.

• Shelf Life:

  2 Years