High-Accuracy Reverse Transcriptase

CAT:
1465-EG21104S
Size:
10000 U
  • Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
  • Dry Ice Shipment: No

High-Accuracy Reverse Transcriptase

  • Description:

    The High-Accuracy Reverse Transcriptase is a modified version of M-MLV GIII Reverse Transcriptase that utilizes nucleic acid aptamer technology. It is covalently linked to the M-MLV enzyme and exhibits suppressed activity below 31°C, thereby enhancing the specificity of the enzyme's products. The optimal reaction temperature for this enzyme is 50°C, and it possesses enhanced extension capabilities, making it suitable for synthesizing longer cDNA fragments.

  • Assay Protocol:

    http://en.best-enzymes.com/storage/upload/files/directions/02nizhuanlu/EG21104.pdf

  • Concentration:

    200 U/μL

  • Components:

    • High-Accuracy Reverse Transcriptase (200 U/μl) : 50 μl • 5x M-MLV First Strand Buffer: 500 μl • 0.1 M DTT: 100 μl

  • Shipping Conditions:

    2~8°C

  • Storage Conditions:

    -20°C

  • Shelf Life:

    2 Years