M-MLV GIV Reverse Transcriptase

• Description:

  M-MLV GIV Reverse Transcriptase is a fourth-generation M-MLV reverse transcriptase obtained through genetic modification and recombination technologies. Compared with the wild-type M-MLV reverse transcriptase, it lacks RNase H activity. Key enhancements include superior thermal stability, with an optimal reaction temperature of 55°C and sustained 50% activity at 70°C. Furthermore, it demonstrates improved tolerance to complex secondary structures , accelerated reaction speed (completing reactions in as little as 10 minutes), and high template affinity. These combined properties ensure robust performance with low-copy templates and enable efficient synthesis of full-length cDNA fragments up to 20 kb.

• Assay Protocol:

  http://en.best-enzymes.com/storage/upload/files/directions/02nizhuanlu/EG24101.pdf

• Concentration:

  200 U/μL

• Components:

  • M-MLV GIV Reverse Transcriptase (200 U/μl) : 50 μl • 5x M-MLV First Strand Buffer: 500 μl • 0.1 M DTT: 100 μl

• Shipping Conditions:

  2~8°C

• Storage Conditions:

  Store at -20°C for 2 years.

• Shelf Life:

  2 Years